pot1 (Novus Biologicals)

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Novus Biologicals pot1
Pot1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pot1/POT1+Antibody/pmc12993239-119-60-62
Average 94 stars, based on 21 article reviews

pot1 - by Bioz Stars, 2026-09

94/100 stars

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Article Title: Positive feedback between p53 and TRF2 in telomere damage signaling and cellular senescence
Article Snippet: Following antibodies were used: anti-TRF2 (1:1,000; 4A794, Upstate, Lake Placid, NY); anti-TRF2 (1:2,000; H-164, Santa Cruz Biotechnology, Santa Cruz, CA); anti-Siah-1 (1:1,000; ab2237, Abcam, Cambridge, MA); anti-p53 (1:2,000; DO-1, Santa Cruz Biotechnology); anti-pS15-p53 (1:1,000; #9284, Cell Signaling Technology, Danvers, MA); anti-p21 WAF1 (1:500; EA10, Calbiochem, San Diego, CA); anti-p16 INK4A (1:1,000; G175-1239, BD Pharmingen, San Diego, CA); anti-RAP1 (1:1,000; Bethyl Laboratories, Montgomery, TX); anti-POT1 (1:1,000; Novus Biologicals, Littleton, CO); anti-TPP1 (1:1,000; Sigma-Aldrich); anti-Siah-2 (1:500; Novus Biologicals); anti-HSP90 (1:2,000; BD Biosciences); anti-Lamin A/C (1:500; Upstate); anti-FLAG tag (1:10,000; M2, Sigma-Aldrich); anti-Myc tag (1:5,000; Invitrogen); anti-HA tag (1:1,000; 3F10, Roche, Indianapolis, IN); anti-GST (1:500; B14, Santa Cruz Biotechnology); anti-His tag (1:3,000; BMG-His-1, Roche); anti-histone H2B (1:2,000; #07-371, Upstate); anti-polyubiquitin (1:500; P4D1, Santa Cruz Biotechnology); anti-β-catenin (1:1,000; BD Biosciences); anti-α-tubulin (1:5,000; Sigma-Aldrich) and anti-β-actin (1:10,000; AC-15, Sigma-Aldrich).

Article Title: Ginsenoside F1-Mediated Telomere Preservation Delays Cellular Senescence.
Article Snippet: The antibodies against RAP1 were obtained from Bethyl laboratories, Inc. (A300306A-9, MA, USA); the anti-TPP1 (ab195234), anti-TRF1 (ab10579), anti-p16 (ab211542), and anti-53BP1 (ab175933) were from Abcam (Cambridge, UK); the anti-POT1 (NB500-176) and anti-TRF2 (NB100-56506) were from Novus Biologicals (Littleton, CO, USA); the antiTIN2 (PA5-67076) was from Invitrogen (Waltham, MA, USA); the anti-p53 (P6874), p16 (ab211542), and anti-actin (A5441) were from Sigma-Aldrich (Darmstadt, Germany); and the anti-P21 (2947), PINK1 (6946), Parkin (4211), autophagy sampler kit (4445), SirT3 (2627), SirT5 (8779), and Tom20 (42406) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Article Title: Positive feedback between p53 and TRF2 in telomere damage signaling and cellular senescence
Article Snippet: Antibodies Following antibodies were used: anti-TRF2 (1:1,000; 4A794, Upstate, Lake Placid, NY); anti-TRF2 (1:2,000; H-164, Santa Cruz Biotechnology, Santa Cruz, CA); anti-Siah-1 (1:1,000; ab2237, Abcam, Cambridge, MA); anti-p53 (1:2,000; DO-1, Santa Cruz Biotechnology); anti-pS15-p53 (1:1,000; #9284, Cell Signaling Technology, Danvers, MA); anti-p21 WAF1 (1:500; EA10, Calbiochem, San Diego, CA); anti-p16 INK4A (1:1,000; G175-1239, BD Pharmingen, San Diego, CA); anti-RAP1 (1:1,000; Bethyl Laboratories, Montgomery, TX); anti-POT1 (1:1,000; Novus Biologicals, Littleton, CO); anti-TPP1 (1:1,000; Sigma-Aldrich); anti-Siah-2 (1:500; Novus Biologicals); anti-HSP90 (1:2,000; BD Biosciences); anti-Lamin A/C (1:500; Upstate); anti-FLAG tag (1:10,000; M2, Sigma-Aldrich); anti-Myc tag (1:5,000; Invitrogen); anti-HA tag (1:1,000; 3F10, Roche, Indianapolis, IN); anti-GST (1:500; B14, Santa Cruz Biotechnology); anti-His tag (1:3,000; BMG-His-1, Roche); anti-histone H2B (1:2,000; #07-371, Upstate); anti-polyubiquitin (1:500; P4D1, Santa Cruz Biotechnology); anti-β-catenin (1:1,000; BD Biosciences); anti-α-tubulin (1:5,000; Sigma-Aldrich) and anti-β-actin (1:10,000; AC-15, Sigma-Aldrich).

Article Title: Ginsenoside F1-Mediated Telomere Preservation Delays Cellular Senescence
Article Snippet: The antibodies against RAP1 were obtained from Bethyl laboratories, Inc. (A300-306A-9, MA, USA); the anti-TPP1 (ab195234), anti-TRF1 (ab10579), anti-p16 (ab211542), and anti-53BP1 (ab175933) were from Abcam (Cambridge, UK); the anti-POT1 (NB500-176) and anti-TRF2 (NB100-56506) were from Novus Biologicals (Littleton, CO, USA); the anti-TIN2 (PA5-67076) was from Invitrogen (Waltham, MA, USA); the anti-p53 (P6874), p16 (ab211542), and anti-actin (A5441) were from Sigma-Aldrich (Darmstadt, Germany); and the anti-P21 (2947), PINK1 (6946), Parkin (4211), autophagy sampler kit (4445), SirT3 (2627), SirT5 (8779), and Tom20 (42406) were obtained from Cell Signaling Technology (Danvers, MA, USA).

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Article Title: METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability.
Article Snippet: Cells were lysed in SDS lysis buffer [50 mM Tris–HCl (pH 6.8), 2% SDS, 0.1% bromophenol blue, 10% glycerol, 1% -mercaptoethanol] and boiled for 10 min. Then proteins were separated on an SDS–PAGE gel and transferred to a polyvinylidene difluoride membrane (PALL). .. The following antibodies were used for immunoblotting: anti-METTL3 (1:2000, ab195352, Abcam), anti-YTHDC1 (1:2000, ab122340, Abcam), antihistone H3 (1:1000, ab1791, Abcam), anti-TRF1 (1:1000 dilution), anti-TRF2 (1:2000 dilution, 05-513, Merck), anti-POT1 (1:2000 dilution, NB500-176, Novus Biologicals), anti-RNase HI (1:2000, 15606–1-AP, Proteintech), anti-glyceraldehydephosphate dehydrogenase (GAPDH; 1:5000, 60004–1-Ig, Proteintech), anti- -tubulin (1:5000, 66031–1-Ig, Proteintech), HRP-conjugated goat anti-rabbit (KPL, Inc.) or goat anti-mouse (KPL, Inc.). ..

Article Title: Distinct mechanisms underlying extrachromosomal telomere DNA generation in ALT cancers
Article Snippet: After additional washes with TBS-T, membranes were treated with ECL reagent, and protein signals were detected using the Odyssey Imaging System (LI-COR 2800). .. Following antibodies were used for Western blot: anti-actin (Sigma–Aldrich, A5316), anti-ATRX (Santa Cruz, sc-15408), anti-DAXX (Santa Cruz, sc-7152), anti-Lamin A/C (Santa Cruz, sc-376248), anti-STN1 (Abcam, ab89250), anti-SMARCAL1 (Santa Cruz, sc- 376 377), anti-RAD51 (Santa Cruz, sc-8349), anti-RPA32 (Abcam, ab2175), anti-RPA32 pS33 (Bethyl, A300-246), anti-FANCM (Novus, NBP2-50418; CV5.1), anti-HLTF (Proteintech, 14286–1-AP), anti-ZRANB3 (Thermo, PA565143 ), anti-RECQ5 (Proteintech, 12468–2-AP), anti-BLM (Bethyl, A300-110), anti-PML (Bethyl, A301-167A), anti-POT1 (Novus, NB500-176), and anti-POLD3 (Proteintech, 21935–1-AP). .. Total RNA was isolated using the Direct-zol RNA Miniprep Kit (ZYMO) according to the manufacturer's instructions. cDNA synthesis was performed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific).

Article Title: METTL3-mediated m 6 A modification stabilizes TERRA and maintains telomere stability
Article Snippet: Cells were lysed in SDS lysis buffer [50 mM Tris–HCl (pH 6.8), 2% SDS, 0.1% bromophenol blue, 10% glycerol, 1% β-mercaptoethanol] and boiled for 10 min. Then proteins were separated on an SDS–PAGE gel and transferred to a polyvinylidene difluoride membrane (PALL). .. The following antibodies were used for immunoblotting: anti-METTL3 (1:2000, ab195352, Abcam), anti-YTHDC1 (1:2000, ab122340, Abcam), anti-histone H3 (1:1000, ab1791, Abcam), anti-TRF1 (1:1000 dilution), anti-TRF2 (1:2000 dilution, 05-513, Merck), anti-POT1 (1:2000 dilution, NB500-176, Novus Biologicals), anti-RNase HI (1:2000, 15606–1-AP, Proteintech), anti-glyceraldehydephosphate dehydrogenase (GAPDH; 1:5000, 60004–1-Ig, Proteintech), anti-α-tubulin (1:5000, 66031–1-Ig, Proteintech), HRP-conjugated goat anti-rabbit (KPL, Inc.) or goat anti-mouse (KPL, Inc.). ..

Expressing:

Article Title: Functional diversity of human protection of telomeres 1 isoforms in telomere protection and cellular senescence.
Article Snippet: .. Antibodies used are as follows: anti-V5 epitope (Invitrogen), anti-myc epitope (Invitrogen), anti-Flag epitope (Sigma-Aldrich), anti-POT1 (Santa Cruz Biotechnology), anti-POT1 (Novus Biologicals), anti-p53 (DO-1, Santa Cruz Biotechnology), anti-phosphop53 (Ser15; Cell Signaling Technology), anti-p21WAF1 (Oncogene Sciences), anti-p16INK4A (Santa Cruz Biotechnology), anti-h-actin (Abcam), anti-gH2AX (Upstate), anti-hRAP1 (Bethyl Laboratories), anti-TRF2 (Santa Cruz Biotechnology), anti-lamin A/C (Santa Cruz Biotechnology), Alexa Fluor 488–conjugated anti-mouse IgG (Molecular Probes), and Alexa Fluor 568–conjugated anti-rabbit IgG (Molecular Probes). mRNA expression analyses. ..

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